PixSeq

A Napari plugin for extracting time series traces from single molecule FRET data.

napari-PixSeq uses Picasso (picassosr) as a backend and includes features for aligning image channels/datasets, undrifting images, detecting/fitting localisations and extracting traces, and supports both ALEX and FRET data. Traces can be exported in different formats for downstream analysis.

napari-PixSeq traces can be analysed with TraceAnalyser: https://github.com/piedrro/TraceAnalyser

This is still undergoing development, so some features may not work as expected.

This was built by Dr Piers Turner from the Kapanidis Lab, University of Oxford.

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This napari plugin was generated with Cookiecutter using @napari's cookiecutter-napari-plugin template.

Installation¶

You can install napari-PixSeq via pip:

pip install napari-PixSeq

You can install napari-PixSeq via [GitHub]:

conda create –-name napari-pixseq python==3.9
conda activate napari-pixseq
conda install -c anaconda git
conda update --all

pip install git+https://github.com/piedrro/napari-PixSeq.git

Contributing¶

Contributions are very welcome. Tests can be run with tox, please ensure the coverage at least stays the same before you submit a pull request.

License¶

Distributed under the terms of the MIT license, "napari-PixSeq" is free and open source software

Issues¶

If you encounter any problems, please file an issue along with a detailed description.