Scalar and vectorial models of the microscope point spread function (PSF).
Python bindings for scalar and vectorial models of the point spread function.
Original C++ code and MATLAB MEX bindings Copyright © 2006-2013, Francois Aguet, distributed under GPL-3.0 license. Python bindings by Talley Lambert
This package contains three models:
- The vectorial model is described in Auget et al 20091. For more information and implementation details, see Francois' Thesis2.
- A scalar model, based on Gibson & Lanni3.
- A gaussian approximation (both paraxial and non-paraxial), using paramters from Zhang et al (2007)4.
pip install psfmodels
git clone https://github.com/tlambert03/PSFmodels.git cd PSFmodels pip install -e ".[dev]" # will compile c code via pybind11
There are two main functions in
Additionally, each version has a helper function called
scalar_psf_centered respectively. The main difference is that the
functions accept a vector of Z positions
zv (relative to coverslip) at which
PSF is calculated. As such, the point source may or may not actually be in the
center of the rendered volume. The
_psf_centered variants, by contrast, do
zv, but rather accept
nz (the number of z planes) and
(the z step size in microns), and always generates an output volume in which the
point source is positioned in the middle of the Z range, with planes equidistant
from each other. All functions accept an argument
pz, specifying the position
of the point source relative to the coverslip. See additional keyword arguments
Note, all output dimensions (
nz) should be odd.
import psfmodels as psfm import matplotlib.pyplot as plt from matplotlib.colors import PowerNorm # generate centered psf with a point source at `pz` microns from coverslip # shape will be (127, 127, 127) psf = psfm.make_psf(127, 127, dxy=0.05, dz=0.05, pz=0) fig, (ax1, ax2) = plt.subplots(1, 2) ax1.imshow(psf[nz//2], norm=PowerNorm(gamma=0.4)) ax2.imshow(psf[:, nx//2], norm=PowerNorm(gamma=0.4)) plt.show()
# instead of nz and dz, you can directly specify a vector of z positions import numpy as np # generate 31 evenly spaced Z positions from -3 to 3 microns psf = psfm.make_psf(np.linspace(-3, 3, 31), nx=127) psf.shape # (31, 127, 127)
all PSF functions accept the following parameters. Units should be provided in microns unless otherwise stated. Python API may change slightly in the future. See function docstrings as well.
nx (int): XY size of output PSF in pixels, must be odd. dxy (float): pixel size in sample space (microns) [default: 0.05] pz (float): depth of point source relative to coverslip (in microns) [default: 0] ti0 (float): working distance of the objective (microns) [default: 150.0] ni0 (float): immersion medium refractive index, design value [default: 1.515] ni (float): immersion medium refractive index, experimental value [default: 1.515] tg0 (float): coverslip thickness, design value (microns) [default: 170.0] tg (float): coverslip thickness, experimental value (microns) [default: 170.0] ng0 (float): coverslip refractive index, design value [default: 1.515] ng (float): coverslip refractive index, experimental value [default: 1.515] ns (float): sample refractive index [default: 1.47] wvl (float): emission wavelength (microns) [default: 0.6] NA (float): numerical aperture [default: 1.4]
Comparison with other models¶
While these models are definitely slower than the one implemented in Li et al (2017) and MicroscPSF, there are some interesting differences between the scalar and vectorial approximations, particularly with higher NA lenses, non-ideal sample refractive index, and increasing spherical aberration with depth from the coverslip.
For an interactive comparison, see the examples.ipynb Jupyter notebook.
Lightsheet PSF utility function¶
psfmodels.tot_psf() function provides a quick way to simulate the total
system PSF (excitation x detection) as might be observed on a light sheet
microscope (currently, only strictly orthogonal illumination and detection are
supported). See the lightsheet.ipynb Jupyter notebook for
- 23 April 2022
- 15 September 2019
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